[Correlation between the property changes of opiate receptors and the structural integrity of synaptosomes]

The effect of incubation of rat brain synaptosomes at 37 degrees C isolated by the Hajos method, on parameters of high affinity opiate receptor binding of [3H]naloxone and the structural integrity of synaptosomes was studied. The structural integrity of synaptosomes was controlled by electron microscopy, by changes in lactate dehydrogenase activity as well as by the ability of synaptosomes to maintain the transmembrane potential, using the fluorescent probe 1-anilino-8-naphthalenesulfonate. It was found that during 60 min incubation, the structure of synaptosomes is considerably impaired. The dynamics of changes in the structural integrity of synaptosomes is correlated with the changes of parameters of naloxone binding to opiate receptors, which points to the dependence of synaptosomal receptors' properties on preparation nativity degree.