Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Atypical deletions generated by mutated IS102 elements.

F Bernardi, A Bernardi

    Molecular & General Genetics : MGG
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Geminivirus replication: rolling circle mechanism in plants].

    Virologie (Montrouge, France)·2022
    Same author

    [Nanoviruses, small plant viruses: similarities and differences with geminiviruses].

    Virologie (Montrouge, France)·2021
    Same author

    Probabilistic tsunami forecasting for early warning.

    Nature communications·2021
    Same author

    Plasma levels of protein C pathway proteins and brain magnetic resonance imaging volumes in multiple sclerosis.

    European journal of neurology·2019
    Same author

    The chaperone-like sodium phenylbutyrate improves factor IX intracellular trafficking and activity impaired by the frequent p.R294Q mutation.

    Journal of thrombosis and haemostasis : JTH·2018
    Same author

    Hemostasis biomarkers in multiple sclerosis.

    European journal of neurology·2018
    Same journal

    A multigene family in Calothrix sp. PCC 7601 encodes phycocyanin, the major component of the cyanobacterial light-harvesting antenna

    Molecular & general genetics : MGG·2017
    Same journal

    Mutants of Schizosaccharomyces pombe which sporulate in the haploid state.

    Molecular & general genetics : MGG·2015
    Same journal

    The chloroplast genomes of conifers lack one of the rRNA-encoding inverted repeats.

    Molecular & general genetics : MGG·2014
    Same journal

    Multiple mutant analysis of recombination in yeast.

    Molecular & general genetics : MGG·2012
    Same journal

    Intergenic suppression of spoO phenotypes by the Bacillus subtilis mutation rvtA.

    Molecular & general genetics : MGG·2010
    Same journal

    Expression of the seqA gene is negatively modulated by the HU protein in Escherichia coli.

    Molecular & general genetics : MGG·2001
    See all related articles

    Mutations in the IS102 element

    Area of Science:

    • Molecular Biology
    • Genetics
    • Microbiology

    Background:

    • The IS102 element is known to potentially encode two polypeptide chains.
    • Understanding the function of these polypeptide chains is crucial for comprehending transposition mechanisms.

    Purpose of the Study:

    • To investigate the role of the larger polypeptide chain encoded by IS102.
    • To characterize the impact of mutations near the COOH terminus on deletion formation.

    Main Methods:

    • Introduction of specific mutations into the larger polypeptide chain of IS102.
    • Assay of residual deletion generation ability in mutated IS102 elements.
    • Analysis of deletion endpoints and structural features.

    Main Results:

    Related Experiment Videos

    • Mutated IS102 elements exhibit a reduced ability to generate deletions.
    • The COOH terminus mutations prevent the recognition of element ends as fixed endpoints for IS-mediated deletions.
    • Some structural features of normal deletion formation remain intact.

    Conclusions:

    • The larger polypeptide chain of IS102 plays a role in recognizing deletion endpoints.
    • The COOH terminus is critical for the proper functioning of IS102 in mediating deletions.
    • IS102-mediated deletion formation is a complex process involving multiple functional domains.