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Related Experiment Videos

Is there a "dopaminergic glial cell"?

E Hansson, L Rönnbäck, A Sellström

    Neurochemical Research
    |May 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Dopamine significantly increases intracellular cyclic adenosine monophosphate (cAMP) in cerebral hemisphere astroglial cells. This effect, dose- and time-dependent, highlights dopamine

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    Area of Science:

    • Neuroscience
    • Cell Biology
    • Biochemistry

    Background:

    • Dopamine signaling plays a crucial role in the central nervous system.
    • Cyclic adenosine monophosphate (cAMP) is a key second messenger involved in various cellular processes.
    • Understanding dopamine's effects on cAMP levels in specific brain cell types is essential for neuroscience research.

    Purpose of the Study:

    • To investigate the effect of dopamine on intracellular cAMP levels in primary cultures of cerebral hemisphere cells.
    • To characterize the dose- and time-dependency of dopamine-induced cAMP elevation.
    • To explore the heterogeneity of astroglial cells in response to dopamine stimulation.

    Main Methods:

    • Primary cultures of cerebral hemisphere cells were incubated with varying concentrations of dopamine.

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  • Apomorphine (partial agonist) and fluphenazine (antagonist) were used to characterize receptor interactions.
  • Propranolol was used to assess potential beta-adrenergic receptor involvement.
  • Immunohistochemistry was employed to identify cell types (S100, GFA proteins).

    • Main Results:

    • Dopamine (10(-4) M) caused a 9-fold increase in intracellular cAMP in cerebral hemisphere cultures.
    • The dopamine effect was dose- and time-dependent.
    • Astroglial cultures from the striatum showed a more pronounced cAMP elevation (12-fold).
    • Dopamine did not stimulate cAMP formation in brain-stem cultures.
    • Fluphenazine partially antagonized the dopamine effect, while propranolol had an incomplete effect.

    Conclusions:

    • Dopamine significantly stimulates cAMP production in astroglial cells from the cerebral hemisphere.
    • The findings suggest dopamine receptor involvement in this signaling pathway.
    • Astroglial cells exhibit heterogeneity in their response to dopamine, particularly between striatal and brain-stem regions.
    • These results contribute to understanding dopamine's role in glial cell function and brain heterogeneity.