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Related Experiment Videos

Staphylococcal enterotoxin A gene is associated with a variable genetic element.

M J Betley, S Löfdahl, B N Kreiswirth

    Proceedings of the National Academy of Sciences of the United States of America
    |August 1, 1984
    PubMed
    Summary
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    The Staphylococcus aureus enterotoxin A (SEA) gene is part of a mobile genetic element. This element

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Staphylococcus aureus produces enterotoxin A (SEA), a key virulence factor.
    • The genetic basis for SEA production in S. aureus has been investigated.

    Purpose of the Study:

    • To clone and characterize the genetic determinant of Staphylococcus aureus enterotoxin A (SEA).
    • To understand the location, structure, and potential mobility of the SEA gene (entA).

    Main Methods:

    • Cloning of the SEA gene (entA) into Escherichia coli using the pBR322 vector.
    • Analysis of the genetic element containing entA, including its size and restriction mapping (HindIII fragment).
    • Investigation of the chromosomal location of the entA element in various S. aureus strains.

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    Main Results:

    • The entA gene was successfully cloned and expressed in E. coli, with SEA secreted into the periplasmic space.
    • The entA gene resides on an 8-12 kilobase-pair genetic element, typically located between purine (pur) and isoleucine-valine (ilv) markers in S. aureus.
    • Variations in the entA element's presence, structure, and location were observed, including complete absence, structural variants, and unlinked copies, suggesting instability and potential mobility.

    Conclusions:

    • The genetic determinant for SEA is associated with a discrete, structurally unstable, and possibly mobile genetic element.
    • The variability in the entA element's location and structure contributes to differences in SEA production among S. aureus strains.