Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Is spectrin a calmodulin-binding protein?

P Boivin, C Galand

    Biochemistry International
    |February 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Calmodulin binds to both alpha and beta spectrin chains individually, but not to intact spectrin dimers. This suggests calmodulin

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Immune adaptive microenvironment profiles in intracerebral and intrasplenic lymphomas share common characteristics.

    Clinical and experimental immunology·2011
    Same author

    Fracture-associated and idiopathic subchondral vertebral lesions: a magnetic resonance study in autopsy specimens with histologic correlation.

    Skeletal radiology·2008
    Same author

    Evidence of the glycation and denaturation of LTP1 during the malting and brewing process.

    Journal of agricultural and food chemistry·2001
    Same author

    Purification and structural characterization of LTP1 polypeptides from beer.

    Journal of agricultural and food chemistry·2000
    Same author

    Pregnancy-induced changes in lower extremity superficial veins: an ultrasound scan study.

    Journal of vascular surgery·2000
    Same author

    Suppression of CDA II expression in a homozygote.

    British journal of haematology·1999
    Same journal

    Monitoring of haemoglobin-methyl isocyanate adduct by high-performance liquid chromatography with diode array detector.

    Biochemistry international·1992
    Same journal

    Intervention of adriamycin induced free radical damage.

    Biochemistry international·1992
    Same journal

    Relationship between substrate activity and pKa value of phenols on sulfotransferase from Eubacterium A-44.

    Biochemistry international·1992
    Same journal

    Purification and properties of chitinase from cabbage.

    Biochemistry international·1992
    Same journal

    Coordinate expression of the enzymes of nitric oxide metabolism in Paracoccus denitrificans.

    Biochemistry international·1992
    Same journal

    Characterization of trehalase in Rhodotorula rubra.

    Biochemistry international·1992
    See all related articles

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Molecular Biology

    Background:

    • Spectrin is a key cytoskeletal protein in human erythrocytes.
    • Calmodulin is a crucial calcium-binding protein involved in various cellular processes.

    Purpose of the Study:

    • To investigate the binding interaction between calmodulin and spectrin from human erythrocytes.
    • To determine if calmodulin binds to spectrin and identify the binding sites.

    Main Methods:

    • Affinity chromatography using a sepharose-calmodulin column.
    • Dissociation of spectrin into alpha and beta chains using 6-7 M urea.
    • Elution of bound proteins using EGTA.

    Main Results:

    • Both alpha and beta spectrin chains bound to the sepharose-calmodulin column with varying affinities.

    Related Experiment Videos

  • Both chains were co-eluted by EGTA, indicating the presence of calmodulin binding sites on both.
  • Intact spectrin dimers did not bind to the column.

    • Conclusions:

    • Calmodulin binding sites exist on both alpha and beta spectrin chains.
    • The lack of binding in intact spectrin dimers suggests a limited physiological role for direct calmodulin-spectrin interaction in erythrocytes.