[Site-specific endonuclease BmeI from Bacillus megaterium 216]

A site-specific endonuclease BmeI has been isolated from Bacillus megaterium 216 by gel filtration on ultragel AcA-44 with a subsequent chromatography on heparin-sepharose 6B. On the double-stranded DNA the endonuclease recognizes the pentanucleotide sequence (Formula: see text); and hydrolyzes it in the points shown by arrows. At gel filtration the endonuclease is eluted in the volume corresponding to a molecular mass of 60 000.