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The structural proteins of chick embryo lethal orphan virus (fowl adenovirus type 1)

P Li, A J Bellett, C R Parish

    The Journal of General Virology
    |October 1, 1984
    PubMed

    Insights

    This study characterizes the structural proteins of Chick embryo lethal orphan (CELO) virus, a fowl adenovirus. Findings reveal distinct viral protein structures and synthesis patterns, aiding in understanding adenovirus biology.

    Area of Science:

    • Virology
    • Molecular Biology
    • Structural Biology

    Background:

    • Chick embryo lethal orphan (CELO) virus, also known as fowl adenovirus type 1, is a significant avian pathogen.
    • Understanding the structural composition of viruses is crucial for comprehending their replication and pathogenesis.

    Purpose of the Study:

    • To present a nomenclature for CELO virus structural polypeptides.
    • To characterize the CELO virus pentons, penton base, and associated fibers.
    • To investigate the synthesis patterns of CELO virion polypeptides.

    Main Methods:

    • Analysis of CELO virus structural proteins using polypeptide molecular weight determination.
    • Isolation and characterization of CELO virus pentons via dialysis.
    • Peptide mapping to compare primary sequences of viral fibers.
    • Monitoring the time course of CELO virion polypeptide synthesis.

    Main Results:

    • CELO virus comprises at least 14 structural proteins ranging from 100K to 6K molecular weight.
    • Penton base (polypeptide III, 92K) and two distinct fibers (long: polypeptide IV, 65K; short: polypeptide VII, 44.5K) were identified and characterized.
    • Peptide mapping indicated no sequence homology between the long and short fibers, suggesting separate genetic origins.
    • CELO virion polypeptides are synthesized late in the viral replication cycle, similar to mammalian adenoviruses.

    Conclusions:

    • A comprehensive nomenclature and molar proportion estimation for CELO virus polypeptides have been established.
    • The structural and genetic distinctness of CELO virus fibers have been elucidated.
    • CELO virus polypeptide synthesis follows a late gene expression strategy, mirroring mammalian adenovirus replication.

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