Correlation of biophysical properties and cell surface antigenic profile of Percoll gradient-separated human natural...

Human peripheral blood nylon wool nonadherent lymphocytes were fractionated according to cellular density on discontinuous Percoll gradients. The various fractions of cells obtained by this procedure were then analyzed to correlate morphology, cytotoxicity, light scatter properties and antigenic profile. The majority of natural killer (NK) cell activity was manifested by light density lymphocytes, which demonstrated relatively high light scatter characteristics, large granular lymphocyte morphology, and expressed the phenotypes: Leu 11+(B73.1+), Leu 7-; Leu 11+(B73.1+), Leu 7+, and Leu 11+(B73.1+), Leu 2a+ (low density), Leu 4-. The denser fractions of Percoll (fractions 3-4) tended to show lower NK activity, fewer large granular lymphocytes, lower light scatter profiles, and to selectively localize the subpopulations of lymphocytes with the phenotypes: Leu 11-, Leu 7+, Leu 4+, and Leu 11-, Leu 7+, Leu 2a+ (high density).