[Multiplication of Aujeszky's disease virus MK-25 on chick fibroblast cell cultures]

Attempts were made to produce multilayer cell cultures of chick fibroblasts in a RC-42 roller as well as to replicate in these the Aujezsky's disease virus. Used were fibroblasts of 10-11-day-old chick embryos. The cells were obtained in a closed system after the classic method with an electromagnetic stirrer and a 0.3 percent trypsin in phosphate buffer, having no Ca and Mg ions. Hank's solution was used as a nutrient growth medium, containing 0.5 per cent lactoalbumin hydrolysate and calf serum at pH 7.4-7.6. Both the cell growth and the cytopathic effect of the virus in the stationary and the roller cultures were carried out simultaneously through microscopy, and the evaluation was made after a fourgrade system. It was found that proper cell cultures or chick fibroblasts could be obtained under the following optimal parameters: volume of nutrient medium--200 cm.3; amount of normal inactivated calf sera--10 per cent; and speed of rotation--10 r. p. m. The titer of the virus was found to surpass by 2 logarithms the virus in the stationary cell cultures for a twice shorter period of cultivation.