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Related Experiment Videos

Dictyostelium transposable element DIRS-1 preferentially inserts into DIRS-1 sequences.

J Cappello, S M Cohen, H F Lodish

    Molecular and Cellular Biology
    |October 1, 1984
    PubMed
    Summary

    Dictyostelium transposable element DIRS-1 frequently inserts into itself, often targeting existing DIRS-1 sequences. These DIRS-1-into-DIRS-1 insertions show minimal sequence alteration and can occur in various orientations.

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Genomics

    Background:

    • Transposable elements (TEs) are mobile DNA sequences that can alter genome structure.
    • Dictyostelium discoideum harbors the unique retrotransposon DIRS-1.
    • Understanding TE insertion mechanisms is crucial for genome stability and evolution.

    Purpose of the Study:

    • To investigate the insertion patterns of the Dictyostelium transposable element DIRS-1.
    • To determine the target specificity and sequence characteristics of DIRS-1 insertions.
    • To explore the implications of DIRS-1 self-insertion on gene expression.

    Main Methods:

    • Sequence analysis of genomic clones containing DIRS-1.
    • Comparison of DIRS-1 terminal repeat sequences with internal DIRS-1 sequences.

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  • Identification and characterization of flanking sequences at insertion sites.
  • Analysis of a cDNA clone derived from a DIRS-1 insertion event.

    • Main Results:

    • Five out of six DIRS-1 insertions occurred within other DIRS-1 sequences (DIRS-1-into-DIRS-1).
    • Flanking sequences at insertion sites were homologous to DIRS-1, with one case showing a complete 4.1-kilobase EcoRI fragment as the target.
    • Insertions exhibited colinear or inverted orientations with minimal duplication or deletion of DIRS-1 sequences.
    • A cDNA clone suggested transcription from a DIRS-1-into-DIRS-1 junction, potentially involving heat-shock promoters.

    Conclusions:

    • DIRS-1 displays a strong propensity for self-insertion into existing DIRS-1 elements.
    • The insertion mechanism does not require intact target DIRS-1 elements and tolerates different orientations.
    • DIRS-1 self-insertion events may influence the expression of nearby genes through promoter elements.