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Related Experiment Videos

Cellular immortalization by a cDNA clone encoding the transformation-associated phosphoprotein p53.

J R Jenkins, K Rudge, G A Currie

    Nature
    |December 13, 1984
    PubMed
    Summary

    Introducing the tumor suppressor protein p53, this study reveals its role in cellular immortality. Transfecting p53 into cells grants them immortality and makes them susceptible to ras oncogene-induced transformation.

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    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Oncology

    Background:

    • Malignant transformation requires at least two cellular alterations: immortality and transformation.
    • Cellular immortality can be induced by carcinogens or specific oncogenes.
    • The role of the tumor suppressor protein p53, often elevated in transformed cells, remains unclear.

    Purpose of the Study:

    • To investigate the role of the murine p53 protein in cellular transformation.
    • To determine if p53 can induce cellular immortality and susceptibility to oncogenes.

    Main Methods:

    • Cloning complementary DNA sequences encoding murine p53.
    • Transfecting p53 expression constructs into primary cells with finite lifespans.
    • Assessing cellular immortality and susceptibility to ras oncogene transformation.

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    Main Results:

    • Transfection of p53 expression constructs induced cellular immortality in cells with finite lifespans.
    • Cells expressing p53 became susceptible to transformation by a ras oncogene.
    • p53 plays a critical role in overcoming cellular senescence.

    Conclusions:

    • The tumor suppressor protein p53 is a key factor in achieving cellular immortality.
    • p53 cooperates with oncogenes like ras to drive malignant transformation.
    • Understanding p53's function provides insights into cancer development and potential therapeutic targets.