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Molecular cloning of a human immunoglobulin lambda chain variable sequence.

Y Tsujimoto, C M Croce

    Nucleic Acids Research
    |November 26, 1984
    PubMed
    Summary
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    Researchers cloned a human V lambda cDNA sequence from Burkitt lymphoma cells. This sequence is related to the NEW V lambda protein and found amplified in specific leukemia cells.

    Area of Science:

    • Immunogenetics
    • Molecular Biology
    • Oncology

    Background:

    • Immunoglobulin lambda light chains are crucial for B-cell receptor diversity.
    • Understanding V lambda gene structure and expression is vital for B-cell malignancies.
    • Previous studies identified distinct V lambda subgroups, including subgroup I.

    Purpose of the Study:

    • To clone and characterize a human V lambda cDNA sequence from a lambda-producing Burkitt lymphoma cell line.
    • To investigate the relationship of the cloned V lambda sequence to known V lambda proteins.
    • To analyze the V lambda gene repertoire and identify potential amplifications in human DNA.

    Main Methods:

    • Cloning of human V lambda cDNA using a constant region gene primer.
    • Nucleotide sequencing and amino acid sequence deduction.

    Related Experiment Videos

  • Southern blot hybridization using a V lambda I probe on human DNA.

    • Main Results:

    • A human V lambda cDNA sequence was successfully cloned from Burkitt lymphoma BL2 cells.
    • The deduced amino acid sequence showed high homology to the NEW V lambda protein of subgroup I.
    • Southern blot analysis revealed at least 12 hybridizing V lambda fragments in human DNA.
    • These V lambda fragments were found to be amplified in K562 cells, which also showed amplified C lambda sequences.

    Conclusions:

    • The study successfully cloned and characterized a novel human V lambda cDNA sequence.
    • The findings suggest a conserved V lambda I subgroup structure and potential gene amplification in certain leukemias.
    • Amplification of V lambda and C lambda sequences in K562 cells warrants further investigation into their role in chronic myelogenous leukemia.