Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Binary Agrobacterium vectors for plant transformation.

M Bevan

    Nucleic Acids Research
    |November 26, 1984
    PubMed
    Summary
    This summary is machine-generated.

    A novel plant transformation vector was developed for efficient gene transfer into plant genomes. This vector uses Agrobacterium tumefaciens functions and includes selectable markers for improved plant genetic engineering.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Cross-sectional study of the prevalence, causes and management of hospital-onset diarrhoea.

    The Journal of hospital infection·2019
    Same author

    The interaction of auxin and cytokinin in the induction of phenylalanine ammonia-lyase in suspension cultures of Phaseolus vulgaris.

    Planta·2013
    Same author

    Subculture-induced protein synthesis in tissue cultures of Glycine max and Phaseolus vulgaris.

    Planta·2013
    Same author

    Some rapid effects of synthetic auxins on mRNA levels in cultured plant cells.

    Planta·2013
    Same author

    Genetic transformation of apple (Malus pumila Mill.) using a disarmed Ti-binary vector.

    Plant cell reports·2013
    Same author

    Genetic transformation in two potato cultivars with T-DNA from disarmed Agrobacterium.

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
    Same journal

    Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

    Nucleic acids research·2026
    Same journal

    Correction to 'Differentiable partition function calculation for RNA'.

    Nucleic acids research·2026
    Same journal

    Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

    Nucleic acids research·2026
    Same journal

    Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

    Nucleic acids research·2026
    Same journal

    Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

    Nucleic acids research·2026
    Same journal

    Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

    Nucleic acids research·2026
    See all related articles

    Area of Science:

    • Plant Biotechnology
    • Molecular Biology
    • Genetic Engineering

    Background:

    • Efficient transformation of higher plants is crucial for genetic improvement.
    • Existing methods often face challenges in efficiency and usability.
    • Agrobacterium tumefaciens-mediated transformation is a widely used technique.

    Purpose of the Study:

    • To construct a highly efficient and user-friendly vector molecule for plant transformation.
    • To leverage the vir region of Ti plasmids for enhanced DNA transfer.
    • To incorporate features facilitating positive selection of inserted DNA.

    Main Methods:

    • Construction of a novel vector molecule.
    • Utilizing trans-acting functions of the vir region of a Ti plasmid.

    Related Experiment Videos

  • Incorporation of T-DNA border sequences for targeted transfer.
  • Introduction of a dominant selectable marker (kanamycin resistance).
  • Inclusion of an M13mp19 lac alpha-complementing region with unique restriction sites.

    • Main Results:

    • The constructed vector enables efficient transfer of sequences into the plant nuclear genome.
    • The vector contains a dominant selectable marker conferring high kanamycin resistance.
    • The lac alpha-complementing region allows for positive selection of inserted DNA via unique restriction sites.

    Conclusions:

    • The developed vector represents an improvement for efficient plant transformation.
    • This tool facilitates enhanced genetic engineering in higher plants.
    • The vector's features simplify the selection of successfully transformed plant cells.