[Isolation of nonintegrated forms of proviral DNA of oncogenic viruses using preparative electrophoresis]

Nonintegrated forms of avian sarcoma virus (ASV) D6 proviral DNA have been analyzed in transformed chick embryo fibroblasts (CEF) 72 h after the initial infection. It is shown that nonintegrated proviral DNA in CEF cultures are represented by circular (form I) and linear (form III) forms. The molecular mass of the linear form of ASV D6 proviral DNA is 5.4-5.6 mD. The new method of preparative electrophoresis is proposed for isolation and separation of the nonintegrated forms of ASV D6 proviral DNA. The advantages of this method are considered as compared to the previously used one.