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Nucleotide sequence of the transposable element IS15.

P Trieu-Cuot, P Courvalin

    Gene
    |October 1, 1984
    PubMed
    Summary

    We sequenced insertion sequence IS15-R, finding it contains a smaller element, IS15-delta, integrated into itself. This novel structure, IS15-R, has implications for understanding transposition and genetic element evolution.

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Genomics

    Background:

    • Insertion sequences (IS) are mobile genetic elements that play a role in genome evolution.
    • The composite transposon Tn1525 is flanked by two copies of IS15.
    • Understanding the structure and function of IS elements is crucial for comprehending genetic plasticity.

    Purpose of the Study:

    • To determine the complete nucleotide sequence of the right (R) copy of insertion sequence IS15.
    • To analyze the structural organization and potential genetic elements within IS15-R.
    • To investigate the relationship between IS15-R and its potential parental element, IS15-delta.

    Main Methods:

    • Complete nucleotide sequencing of the IS15-R element.
    • Bioinformatic analysis of the DNA sequence to identify open reading frames (ORFs) and structural features.
    • Comparative analysis of IS15-R with related genetic elements.

    Main Results:

    • The complete nucleotide sequence of IS15-R (1648 bp) was determined.
    • IS15-R contains short (14 bp) perfect inverted repeats at its termini.
    • Sequence analysis revealed that IS15-R is formed by the integration of a smaller element, IS15-delta (820 bp), into itself, with an 8 bp duplication in the target DNA.
    • IS15-delta has two large overlapping ORFs on opposite strands, resulting in IS15-R having four large ORFs with variations from the parental element.

    Conclusions:

    • IS15-R is a composite insertion sequence formed by the self-integration of IS15-delta.
    • The unique structure of IS15-R, with four large ORFs, suggests potential for novel functions.
    • This finding provides insights into the mechanisms of IS element formation and evolution through internal transposition events.

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