Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Efficient Bacillus subtilis cloning system using bacteriophage vector phi 105J9.

J Errington

    Journal of General Microbiology
    |October 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Population pharmacokinetics of carboplatin, etoposide and melphalan in children: a re-evaluation of paediatric dosing formulas for carboplatin in patients with normal or mild impairment of renal function.

    British journal of clinical pharmacology·2018
    Same author

    Pooling of genital swabs for detection by PCR of Taylorella equigenitalis, the cause of contagious equine metritis.

    Equine veterinary journal·2018
    Same author

    Increased phylogenetic diversity of bovine viral diarrhoea virus type 1 isolates in England and Wales since 2001.

    Veterinary microbiology·2012
    Same author

    Prevalence of Coxiella burnetii in livestock abortion material using PCR.

    The Veterinary record·2011
    Same author

    Development and validation of RT-PCR tests for the detection and S1 genotyping of infectious bronchitis virus and other closely related gammacoronaviruses within clinical samples.

    Transboundary and emerging diseases·2011
    Same author

    Detection of IBV QX in commercial broiler flocks in the UK.

    The Veterinary record·2011
    Same journal

    Osmoregulation in Azospirillum brasilense: glycine betaine transport enhances growth and nitrogen fixation under salt stress.

    Journal of general microbiology·2012
    Same journal

    Glutamine metabolism during aerial mycelium growth of Neurospora crassa.

    Journal of general microbiology·2011
    Same journal

    Glutamine requirement for aerial mycelium growth in Neurospora crassa.

    Journal of general microbiology·2011
    Same journal

    Heat-sensitive lysis mutants of Bacillus subtilis 168 blocked at three different stages of peptidoglycan synthesis.

    Journal of general microbiology·2010
    Same journal

    The lecithinase of Clostridium bifermentans and its relation to the alpha-toxin of Clostridium welchii.

    Journal of general microbiology·2010
    Same journal

    The production by certain species of Clostridium of enzymes disintegrating hide powder.

    Journal of general microbiology·2010
    See all related articles

    A new bacteriophage vector, phi 105J9, enables efficient gene cloning in Bacillus subtilis. This system allows for the isolation of sporulation and biosynthetic genes, offering an alternative to Escherichia coli-based methods.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Bacillus subtilis is a key organism in industrial biotechnology and a model for studying Gram-positive bacteria.
    • Existing cloning systems in B. subtilis have limitations, driving the need for improved methods.

    Purpose of the Study:

    • To develop and characterize a novel bacteriophage vector for efficient gene cloning in Bacillus subtilis.
    • To demonstrate the utility of the new vector for isolating functional genes from B. subtilis.

    Main Methods:

    • Construction of the bacteriophage phi 105J9 vector with specific enzyme cloning sites (BamH1, XbaI, SalI).
    • Transfection of B. subtilis protoplasts with the vector in the presence of polyethylene glycol.
    • Construction of genomic libraries and isolation of target genes.

    Related Experiment Videos

    Main Results:

    • The phi 105J9 vector efficiently accommodates DNA inserts up to 4 kbp.
    • Recombinant phages exhibit normal plaque formation and lysogenization.
    • Successfully isolated functional sporulation and biosynthetic genes from B. subtilis libraries.

    Conclusions:

    • The phi 105J9 vector provides an efficient and versatile system for cloning in Bacillus subtilis.
    • This system offers a valuable alternative to current cloning strategies, particularly those relying on Escherichia coli.