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Updated: Oct 21, 2026

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
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An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

The complete sequence of a full length cDNA for human liver glyceraldehyde-3-phosphate dehydrogenase: evidence for

P Arcari, R Martinelli, F Salvatore

    Nucleic Acids Research
    |December 11, 1984
    PubMed

    Insights

    Researchers identified a glyceraldehyde-3-phosphate dehydrogenase (G3PD) gene clone (pG1) from human liver cDNA. This clone showed higher expression in cancer cell lines, suggesting G3PD

    Area of Science:

    • Molecular Biology
    • Human Genetics
    • Biochemistry

    Background:

    • Glyceraldehyde-3-phosphate dehydrogenase (G3PD) is a key enzyme in glycolysis.
    • Understanding G3PD gene expression is crucial for cellular metabolism research.
    • Previous studies suggested potential variations in G3PD genes.

    Purpose of the Study:

    • To isolate and sequence a full-length human G3PD cDNA clone.
    • To investigate the expression patterns of G3PD in different human tissues and cell lines.
    • To explore the genetic basis and potential multiplicity of human G3PD genes.

    Main Methods:

    • Isolation of a human fetal liver cDNA fragment (clone O42).
    • Screening of a human adult liver cDNA library to obtain a full-length clone (pG1).
    • Complete sequencing of clone pG1 using the chain termination method.
    • RNA hybridization (Northern blot) to analyze G3PD expression.
    • DNA Southern blot analysis to assess G3PD gene copy number.

    Main Results:

    • A 1008 bp coding region and flanking sequences of human G3PD cDNA (clone pG1) were sequenced.
    • Clone pG1 hybridized to a single band in human liver RNA, but showed stronger signals in hepatocarcinoma and kidney carcinoma cell lines.
    • Sequence homology analysis indicated the existence of multiple G3PD genes in the human genome.
    • Southern blot analysis confirmed the presence of several homologous G3PD sequences in human DNA.

    Conclusions:

    • The study successfully isolated and sequenced a full-length human G3PD cDNA.
    • Differential expression of G3PD was observed, with higher levels in certain cancer cell lines.
    • Evidence strongly suggests the presence of multiple G3PD genes in the human genome.

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