Related Experiment Videos
Fructose 1, 6 bisphosphatase activities in mouse liver as a function of age
Insights
Mouse liver fructose 1, 6 bisphosphatase activity shows a peak at 7 days, then declines by 30 days. Both neutral and alkaline forms of the enzyme are present during this developmental period.
Area of Science:
- Biochemistry
- Developmental Biology
- Enzymology
Background:
- Fructose 1, 6 bisphosphatase is a key gluconeogenic enzyme.
- Understanding its activity during development is crucial for metabolic studies.
Purpose of the Study:
- To investigate the activity patterns of neutral and alkaline fructose 1, 6 bisphosphatase in mouse liver during postnatal development.
- To determine the optimal pH for enzyme activity across different developmental stages.
Main Methods:
- Enzyme activity assays were performed on mouse liver samples at various postnatal days.
- Activity was measured across a range of pH values to determine optimal conditions.
Main Results:
- Neutral and alkaline fructose 1, 6 bisphosphatase activity increased up to 7 days postpartum, followed by a decline by 30 days.
- Optimal activity for both enzyme forms was observed at pH 6.5 and 9.0 throughout the study period.
Conclusions:
- The presence of both neutral and alkaline fructose 1, 6 bisphosphatase in mouse liver suggests distinct roles during development.
- The enzyme likely maintains similar properties across the studied developmental stages, indicated by consistent optimal pH.
Abstract:
Neutral fructose 1, 6 bisphosphatase activity increases till 7 days, after which, a decline is observed postnatally upto 30 days. Alkaline fructose 1, 6 bisphosphatase follows the same pattern. The optimum activity of fructose 1, 6 bisphosphatase in mouse liver at pH 6.5 and 9.0 of all the periods, suggests the presence of both neutral and alkaline enzyme during the developmental period studied. On the basis of similarity observed in optimum pH, the same properties of enzyme at all the developmental stages studied, could not be ruled out.