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Published on: October 16, 2017
Murine teratocarcinoma: a model for virus-cell interaction in a differentiating cell system
1Department of Pathology, University of Colorado School of Medicine, Denver 80262.
Journal of Supramolecular Structure and Cellular Biochemistry
|January 1, 1981
Summary
Murine teratocarcinoma stem cells resist Simian virus 40 (SV40) infection. Attempts to modify infection procedures, including co-infection with adenovirus 5, failed to induce viral T-antigen expression or DNA replication.
Area of Science:
- Virology
- Cell Biology
- Cancer Research
Background:
- Murine teratocarcinoma stem cells are resistant to Simian virus 40 (SV40) and polyoma virus infection.
- Understanding viral entry and replication blocks in stem cells is crucial for virology and cancer research.
Purpose of the Study:
- To investigate methods for overcoming the viral infection block in murine teratocarcinoma stem cells.
- To determine if SV40 T-antigen expression and viral DNA replication can be induced in these cells.
Main Methods:
- Cells were subjected to multiple infections with high-titer SV40.
- Pretreatment with DEAE-dextran or 4-nitroquinoline 1-oxide was performed.
- Co-infection with adenovirus 5 and SV40 was utilized.
- Immunofluorescence and immunoprecipitation assays were used to detect SV40 T-antigen.
- Viral DNA replication was assessed by UV irradiation sensitivity in the presence of 5-bromodeoxyuridine.
Main Results:
- Multiple infections and pretreatments did not induce SV40 T-antigen production.
- Co-infection with adenovirus 5 did not overcome the block to SV40 T-antigen expression.
- Neither immunofluorescence nor immunoprecipitation detected T-antigen in infected cells.
- Viral DNA within the embryonal carcinoma cells did not demonstrate replication.
Conclusions:
- The block to SV40 infection in murine teratocarcinoma stem cells is robust and not overcome by tested procedures.
- These findings suggest a fundamental incompatibility between SV40 and these specific stem cells, preventing viral replication and T-antigen expression.
