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Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
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[Enzyme immunoassay in the detection of Clostridium perfringens enterotoxin]

D Dobosch1

  • 1Centers for Disease Control, Atlanta, Ga.

Revista Argentina De Microbiologia
|January 1, 1984
PubMed

Abstract:

An enzyme-linked immunosorbent assay (ELISA) for C. perfringens enterotoxin detection was evaluated using purified enterotoxin preparations, supernatant fluids of sporulating C. perfringens cultures and other Clostridium species. The assay was found satisfactorily sensitive and specific to detect C. perfringens enterotoxin.

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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