[The methyleneblue reduction disc test (M.R.D.-test): a simplified method for the identification of Pseudomonas...

The identification of Pseudomonas species in practice can be improved by testing the utilization of different organic compounds as single sources of carbon. The introduction of this kind of technique into routine laboratory work requires a simplification of the test system. In order to meet these requirements the methyleneblue reduction disc test (M.R.D.-test) had been developed. In this test discs loaded with different organic compounds are introduced into suspensions of the strain examined as single source of carbon. Before to these bacterial suspensions a mineral salts solution containing methyleneblue as a redox indicator had been added. With the help of this method the utilization of a large number of different organic compounds as single sources of carbon can be tested in a way which is neither laborious nor time consuming. The disc once prepared can be stored for some time, single strains can be tested without any difficulty as they are isolated during routine laboratory work.