Freeing rabies virus of the neuroallergenic factor from brain tissue

A method of fixed rabies virus purification from infected sheep brains was proposed. It consisted of suspending the brain tissue in phosphate-buffered hypertonic (0.3 M) NaCl solution, shaking at 37 degrees C and low speed centrifugation at the same temperature. From 65 to 85% ballast proteins were removed, the neuroallergenic activity of the material was lost, but practically no losses of the virus occurred.