Chloride ions enhance L-glutamate binding to rat brain synaptic membranes

Chloride ions increased L-glutamate (L-Glu) binding to synaptic membranes. The binding was saturable and resulted in a 2.5-fold enhancement at concentrations of 20--40 mM chloride. Sodium and potassium ions inhibited only chloride stimulated L-Glu binding. Calcium ions also increased L-Glu binding but this was observed only in the presence of chloride. The anion selectivity of the enhancement of L-Glu binding was similar to that reported for the membrane chloride channel, suggesting that some L-Glu binding sites may be associated with this channel.