A bacteriocin typing scheme for Bacteroides

Epidemiological studies of Bacteroides spp. have been hindered because a suitable typing method is not available. In preliminary studies, 50 strains of Bacteroides were screened against each other for bacteriocin production and sensitivity; 54% of them produced bacteriocin(s) and more than 90% were sensitive to at least one bacteriocin. After calculation of similarity values for these 50 isolates, a typing set of six bacteriocinogenic strains was selected for a typing method based on bacteriocin sensitivity. With this typing set c. 90% of strains could be typed and tests of reproducibility suggested that acceptable accuracy and discrimination could be obtained without applying any one-reaction or two-reaction difference rules. Isolates from four hospitals gave a similar spectrum of typing patterns with 18 bacteriocin types being demonstrated. There was not correlation between bacteriocin type and species of Bacteroides.