Assignment of reovirus mutant group 'G' to its genomic segment

Temperature sensitive (ts+) recombinants between the UV-inactivated reovirus type 1 and the group G ts mutant of reovirus type 3 were obtained using a cross-reactivation technique. Analysis of the dsRNA of recombinant clones in PAGE allowed a preliminary mapping of the ts mutation into its genomic S3 segment. This was confirmed by the hybridization test.