Neurotransmitters extraction by local intracerebral dialysis in anesthetized rats

A new technique for in vivo neurotransmitter extraction was developed. Perfusion U shaped cannulae were chronically implanted in the caudate or the thalamus of rats. A segment of the cannula was a piece of dialysis tube of molecular weight cut off 1000. Measurable amounts of dopamine and norepinephrine were recovered from the perfusion fluid. Intraperitoneal amphetamine 10 mg/kg increased dopamine output from the caudate but not from the thalamus. The potential applications of this technique are discussed.