[Influence of the fermentative cross-seams on the structure of the microsomal membrane]

In rat liver microsomes freezing with subsequent thawing led to irreversible redistribution of protein-lipid packing. This redistribution was detected by a change in the efficiency of energy transfer between protein aromatic groups of membrane protein and lipid-soluble fluorescent probe pyrene. Transglutaminase pretreatment of microsomes prevented the irreversible redistribution. The enzyme is shown to bind no more than 15 per cent of the whole membrane protein. This smaller part of the microsomal protein is supposed to play the decisive role in the movements of its remaining part.