Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nuclear transplantation in mouse embryos.

J McGrath, D Solter

    The Journal of Experimental Zoology
    |November 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Nuclear transplantation in mouse embryos reveals developmental capacity of different cell nuclei. This technique also identified stage-specific embryonic antigen 3 as a maternal cytoplasmic factor.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Canadian Surgery Forum: Abstracts of presentations to the Annual Meetings of the Canadian Association of Bariatric Physicians and Surgeons, Canadian Association of General Surgeons, Canadian Association of Thoracic Surgeons, Canadian Hepato-Pancreato-Biliary Association, Canadian Society of Surgical Oncology, Canadian Society of Colon and Rectal Surgeons, Vancouver, BC, Sept. 17-21, 2013.

    Canadian journal of surgery. Journal canadien de chirurgie·2025
    Same author

    Large-scale analysis of structural brain asymmetries during neurodevelopment: Associations with age and sex in 4265 children and adolescents.

    Human brain mapping·2024
    Same author

    Sleep problems in children and adolescents in an attention deficit hyperactivity disorder service.

    Irish journal of psychological medicine·2023
    Same author

    Risk factors for SARS-CoV-2 infection in healthcare workers following an identified nosocomial COVID-19 exposure during waves 1-3 of the pandemic in Ireland.

    Epidemiology and infection·2022
    Same author

    Autism spectrum disorders: current issues and future directions.

    Irish journal of psychological medicine·2022
    Same author

    Antipsychotic prescribing in GMS paediatric and young adult population in Ireland 2005-2015: repeated cross-sectional study.

    Irish journal of psychological medicine·2021

    Area of Science:

    • Developmental Biology
    • Cell Biology
    • Genetics

    Background:

    • Nuclear transplantation assesses nuclear differentiation consequences.
    • It can define the role of maternal cytoplasmic factors in embryogenesis.

    Purpose of the Study:

    • To extend nuclear transplantation techniques for assessing nuclei from various embryonic stages.
    • To determine the cytoplasmic origin of stage-specific embryonic antigen 3 (SSEA-3).

    Main Methods:

    • Efficient transfer of pronuclei and nuclei from two-, four-, eight-cell, and inner cell mass (ICM) stages into enucleated mouse embryos.
    • Utilizing nuclear transplantation to trace the origin of embryonic antigens.

    Main Results:

    • Successfully transferred nuclei from various embryonic stages, including ICM cells.

    Related Experiment Videos

  • Determined that SSEA-3 originates from the unfertilized ovum's cytoplasm.

    • Conclusions:

    • Nuclear transplantation is valuable for assessing the developmental potential of diverse embryonic nuclei.
    • This technique aids in understanding the nuclear/cytoplasmic origins of early embryonic gene products.