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Updated: Aug 4, 2026

Isolation and Activation of Murine Lymphocytes
Published on: October 30, 2016
[Changes in the membrane receptors of lymphocyte populations during immunization]
Abstract:
A population of splenic lymphocytes has been studied as well as their surface antibodies and their affinity for antigen in different days and periods of immunization. Fractionation of a specific population of lymphocytes having an affinity for antigens coated on glass beads were accomplished. The other aspecific population did not bind ot the glass beads and was not retained on the column. During the primary immuno-response the number of lymphocytic cells with surface antibodies specific for antigen was higher than in the booster response: in secondary response only the total number of antibody forming-cells increases while the number of lymphocytes with surface antibodies is constant. The authors suppose that the surface of spleen lymphocytes is modificated in different way at different time, by immunizing antigen.
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