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Effect of immunosuppression on morphological changes in CNS of monkeys infected with different measles virus vaccine
Abstract:
Residual neurovirulence of measles virus vaccine strains "Leningrad-16", "Moscow-5" and of cloned variant of L-16 strain--clone 3, differing in the degree of attenuation, was studied in intracerebrally (i.c.) infected Cercopithecus aethiops monkeys, receiving cortisone and/or antilymphocyte serum (ALS). Whereas the sensitivity for detection of the potential neurovirulence of vaccine strains was not increased by cortisone treatment. ALS had such effect in detection of neurotropic properties of the L-16 strain and its cloned variants.
Insights
Antilymphocyte serum (ALS) enhanced the detection of neurotropic properties in measles virus vaccine strains, specifically Leningrad-16 and its variants, in monkey studies. Cortisone treatment did not increase sensitivity for detecting vaccine strain neurovirulence.
Area of Science:
- Virology
- Immunology
- Neuroscience
Background:
- Measles virus vaccine strains undergo attenuation to reduce pathogenicity.
- Assessing residual neurovirulence is crucial for vaccine safety.
- Immunosuppressive agents can potentially reveal latent neurotropic properties.
Purpose of the Study:
- To evaluate the residual neurovirulence of measles virus vaccine strains Leningrad-16, Moscow-5, and a cloned variant of L-16.
- To determine the effect of cortisone and/or antilymphocyte serum (ALS) on detecting neurovirulence in these vaccine strains.
Main Methods:
- Intracerebral inoculation of Cercopithecus aethiops monkeys with measles virus vaccine strains.
- Administration of cortisone and/or antilymphocyte serum (ALS) to immunosuppress the monkeys.
- Assessment of neurotropic properties and residual neurovirulence.
Main Results:
- Cortisone treatment did not enhance the sensitivity for detecting potential neurovirulence of the studied measles vaccine strains.
- Antilymphocyte serum (ALS) significantly increased the sensitivity for detecting neurotropic properties of the Leningrad-16 strain and its cloned variants.
- The cloned variant of the L-16 strain exhibited detectable neurotropic properties under ALS treatment.
Conclusions:
- Antilymphocyte serum (ALS) is a valuable tool for uncovering subtle neurotropic potential in attenuated measles virus vaccine strains.
- Cortisone is less effective than ALS in revealing residual neurovirulence of these vaccine strains.
- Further investigation into the neurotropic characteristics of measles vaccine strains, particularly under altered immune conditions, is warranted.