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Enteric neural pathways inhibitory to rabbit duodenal serotonin release
Surgery
|August 1, 1984
Summary
A nonadrenergic, noncholinergic neural pathway inhibits duodenal serotonin release. This pathway, primarily on the basal surface, likely uses adenosine triphosphate (ATP) as its neurotransmitter, especially during acid stimulation.
Area of Science:
- Gastroenterology
- Neurogastroenterology
- Cell Physiology
Background:
- Adrenergic and cholinergic pathways influence duodenal serotonin (5-HT) release.
- Luminal acid stimulates duodenal 5-HT release.
- The overall neural control of 5-HT release requires further elucidation.
Purpose of the Study:
- To investigate the overall neural contribution to duodenal 5-HT release.
- To determine the location and neurotransmitter of a potential nonadrenergic, noncholinergic inhibitory pathway.
- To assess the role of adenosine triphosphate (ATP) and vasoactive intestinal polypeptide (VIP) in regulating 5-HT release.
Main Methods:
- Rabbit duodenal mucosa was studied in a modified Ussing chamber.
- Tetrodotoxin (TTX), a nerve-conduction blocker, was applied to mucosal and/or serosal surfaces.
- Serotonin (5-HT) levels were measured using radioimmunoassay at neutral and acidic pH.
Main Results:
- TTX significantly increased 5-HT release when applied to both surfaces or the serosal surface alone.
- TTX applied to the mucosal surface alone reduced acid-stimulated 5-HT release.
- Adenosine triphosphate (ATP) significantly reduced acid-stimulated 5-HT release, while VIP had no effect.
Conclusions:
- A nonadrenergic, noncholinergic neural pathway inhibits duodenal mucosal 5-HT release.
- This inhibitory pathway is predominantly located on the basal surface of mucosal cells.
- ATP is the likely neurotransmitter mediating this inhibitory response.