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[Uptake of acetate by Acinetobacter calcoaceticus]
Acta Biologica Et Medica Germanica
|January 1, 1977
Summary
Acinetobacter calcoaceticus best uptakes acetate when grown on acetate. Early-growth cells exhibit faster acetate transfer than late-growth cells, indicating growth phase impacts nutrient transport.
Area of Science:
- Microbiology
- Biochemistry
Context:
- Acinetobacter calcoaceticus is a versatile bacterium known for its metabolic capabilities.
- Understanding nutrient uptake mechanisms is crucial for microbial physiology and biotechnology.
Purpose:
- To investigate the kinetics of acetate uptake in Acinetobacter calcoaceticus.
- To determine the influence of carbon source and growth phase on acetate transport.
Summary:
- Acetate uptake was optimal in cells grown solely on acetate.
- Early-log phase cells showed twice the acetate transfer rate compared to late-log phase cells.
- Acetate uptake followed biphasic saturation kinetics with distinct Km values (1.10⁻⁵ M and 1.8x10⁻⁴ M) and maximal rates (8 and 37 mM/min/mg dry weight).
- Inhibition studies revealed that alpha-ketoglutarate, fumarate, L-malate, oxalacetate, uranylacetate, respiratory chain inhibitors, and proton conductors affect acetate uptake.
Impact:
- Provides detailed kinetic parameters for acetate transport in Acinetobacter calcoaceticus.
- Highlights the importance of growth phase and carbon source in optimizing microbial nutrient assimilation.
- Offers insights into the regulatory mechanisms and potential inhibitors of acetate uptake, relevant for metabolic engineering and industrial applications.