Purification of radioiodinated somatostatin-related peptides by reversed-phase high-performance liquid chromatography

In order to set up specific radioimmunoassays for the two N- and C-terminal tetradecapeptides of somatostatin-28 the peptides somatostatin SS14 and SS28 and the somatostatin by-products 1-Tyr-SS14, 11-Tyr-14 and desaminotyrosyl-beta-alanine fragment (1----14) of SS28 were radioiodinated by the chloramine-T or Bolton-Hunter techniques. Reversed-phase high-performance liquid chromatography was shown to be a very efficient and reliable method for the purification of different radioactive reaction media. The corresponding labelled peptides were tested for their relative immunological (radioimmunoassay) and biological (binding studies) properties.