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Activation of C1.

M G Colomb, G J Arlaud, C L Villiers

    Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
    |September 6, 1984
    PubMed
    Summary
    This summary is machine-generated.

    The complement system

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    Area of Science:

    • Immunology
    • Biochemistry
    • Structural Biology

    Background:

    • The first component of complement, C1, is a calcium-dependent complex crucial for immune responses.
    • C1 comprises C1q, which binds activators, and C1r2-C1s2, responsible for proteolytic activity.
    • The autoactivation potential of C1 and its regulation are not fully understood.

    Purpose of the Study:

    • To elucidate the structural organization and activation mechanism of the C1 complex.
    • To propose a model for reconstituted soluble C1 based on integrated experimental data.
    • To understand how C1 autoactivation is regulated in vivo.

    Main Methods:

    • Electron microscopy
    • Neutron diffraction
    • Ultra-centrifugation

    Related Experiment Videos

  • Biochemical assays
  • Functional analysis of C1 subcomponents

    • Main Results:

    • A structural model for reconstituted soluble C1 was proposed.
    • The C1r2-C1s2 subunit adopts a flexible, folded conformation around C1q.
    • Catalytic domains of C1r and C1s are positioned within the C1q cone.
    • C1 activation involves the release of negative control and modulation of C1r's autocatalytic potential.

    Conclusions:

    • The study provides a comprehensive model for the structure and activation of the C1 complex.
    • Understanding C1 structure and regulation is key to comprehending complement system function.
    • This work sheds light on the molecular mechanisms underlying immune complex-mediated C1 activation.