The subpopulations of lymph node cells from allograft-sensitized AKR mice

Electrokinetic behaviour of skin allograft-sensitized LNC and their subpopulations from AKR mice were studied. Sensitized LNC showed a significant reduction in the mean EPM in the post-transplantation period. Histogram analysis of both unsensitized and sensitized LNC of AKR mice showed a heterogeneous population. This heterogeneity in the lymph node cells was more pronounced in the case of sensitized cells. While unsensitized cells resolved into two subpopulations, sensitized LNC resolved into three distinct subpopulations. The emergence of a new population in the case of sensitized cells may be a consequence of allograft sensitization. Subpopulations of unsensitized and sensitized LNC were also obtained by surface marker dependent and differential adherence to nylon wool procedures. Histograms of these subpopulations showed heterogeneity, indicating that both T and B lymphocytes are affected by allograft sensitization. These findings were further confirmed by interacting the subpopulations with specific HCA antigen.