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Related Experiment Videos

Interferon production by individual L cells.

G E Brown, E H Simon, C Chung

    The Journal of General Virology
    |March 1, 1980
    PubMed
    Summary
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    Nearly all mouse L cells produce interferon when infected with Newcastle disease virus (NDV). Interferon production varies greatly between cells, and some cells constitutively produce interferon, especially after cell or virus mutation.

    Area of Science:

    • Immunology
    • Virology
    • Cell Biology

    Background:

    • Interferons are crucial antiviral proteins.
    • Understanding interferon induction is key to antiviral research.
    • Newcastle disease virus (NDV) is a known interferon inducer.

    Purpose of the Study:

    • To determine the proportion of mouse L cells capable of interferon production upon NDV infection.
    • To investigate factors influencing interferon production variability at the single-cell level.
    • To assess the role of viral infectivity and cell mutation in interferon production.

    Main Methods:

    • Utilized a protected center technique with agarose to isolate interferon-producing cells.
    • Infected mouse L cells with Newcastle disease virus (NDV).

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  • Examined interferon production in cells exposed to u.v.-irradiated virus and mutagens.

    • Main Results:

    • Essentially all mouse L cells can be induced to produce interferon by NDV.
    • Significant variability in interferon production was observed among individual cells.
    • U.v.-irradiated NDV lost its interferon-inducing capacity at a rate similar to its infectivity loss.
    • A small fraction of cells constitutively produced interferon, with this fraction increasing after cell or virus u.v. irradiation or chemical mutagenesis.

    Conclusions:

    • Mouse L cells demonstrate a high capacity for interferon induction upon viral challenge.
    • Single-cell analysis reveals heterogeneity in cellular responses to viral infection.
    • Cellular and viral factors, including DNA damage, can modulate interferon production and constitutive expression.