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An established routine method for differential staining of epoxy-embedded tissue sections
Microscopica Acta
|May 1, 1980
Summary
This study presents a simple staining method for epoxy-embedded tissues, highlighting PAS-positive structures in red against a blue background. This technique offers excellent contrast and durability for microscopy and photography.
Area of Science:
- Histology
- Microscopy Techniques
- Cell Biology
Background:
- Effective staining is crucial for visualizing cellular and tissue structures.
- Existing methods for semithin epoxy-embedded sections may lack contrast or long-term stability.
Purpose of the Study:
- To introduce a novel, simple, and effective staining method for semithin epoxy-embedded sections.
- To provide vivid differential staining for improved tissue orientation and microphotography.
Main Methods:
- Utilizes periodic acid oxidation, Schiff's reagent, and methylene blue/azure II counter-staining.
- Optimized for 1-2 micron thick Epon sections with specific incubation times and temperatures.
Main Results:
- Achieves specific red staining of PAS-positive structures against differential blue staining of other components.
- Demonstrates excellent contrast and stability, with no fading observed over 10 years.
- Suitable for a variety of tissues.
Conclusions:
- The described staining method is simple, effective, and provides brilliant differential staining.
- Recommended for tissue orientation in electron microscopy and for high-quality microphotography.
- Offers a durable and visually appealing staining solution for biological samples.