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Related Experiment Videos

Human leukocyte interferon produced by E. coli is biologically active.

D V Goeddel, E Yelverton, A Ullrich

    Nature
    |October 2, 1980
    PubMed
    Summary

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    Human leukocyte interferon was synthesized and cloned in E. coli, producing high yields. This recombinant interferon demonstrated protective effects against lethal viral infections in animal models.

    Area of Science:

    • Biotechnology
    • Molecular Biology
    • Immunology

    Background:

    • Interferons are crucial antiviral proteins.
    • Recombinant DNA technology enables large-scale protein production.
    • Viral infections pose significant health challenges.

    Purpose of the Study:

    • To synthesize and clone human leukocyte interferon cDNA.
    • To express functional interferon in Escherichia coli.
    • To evaluate the antiviral efficacy of the produced interferon.

    Main Methods:

    • Enzymatic synthesis of human leukocyte interferon cDNA.
    • Cloning into the pBR322 vector and transformation of E. coli.
    • Construction of an expression plasmid for high-level interferon production.

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    Main Results:

    • The DNA sequence encoded a signal peptide and a 165-amino acid interferon polypeptide.
    • E. coli produced 2.5 x 10(8) units of interferon per liter.
    • The synthesized interferon (LeIF) protected squirrel monkeys from encephalomyocarditis virus.

    Conclusions:

    • Successful synthesis and high-yield expression of human leukocyte interferon in E. coli.
    • Demonstrated in vivo antiviral activity of recombinant human leukocyte interferon.
    • Potential therapeutic applications of engineered interferons against viral diseases.