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Le interferon mRNA from human fibroblasts.

R H Pang, T G Hayes, J Vilcek

    Proceedings of the National Academy of Sciences of the United States of America
    |September 1, 1980
    PubMed
    Summary
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    Human F and Le interferons exhibit distinct properties. Their differential production in fibroblasts, triggered by Newcastle disease virus (NDV) or poly(I)·poly(C), is regulated at the messenger RNA (mRNA) level.

    Area of Science:

    • Immunology
    • Molecular Biology
    • Virology

    Background:

    • Human interferons F and Le are distinct antiviral proteins with different antigenic properties and host ranges.
    • Understanding the regulation of interferon production is crucial for developing effective antiviral therapies.

    Purpose of the Study:

    • To investigate the molecular mechanisms underlying the differential induction of human F and Le interferon synthesis.
    • To determine if the regulation occurs at the level of messenger RNA (mRNA) or protein synthesis.

    Main Methods:

    • GM-258 fibroblasts were stimulated with Newcastle disease virus (NDV) or poly(I)·poly(C).
    • Polyadenylylated mRNA was isolated from stimulated cells and injected into Xenopus laevis oocytes.
    • Interferon activities produced in oocytes were analyzed and compared to those in intact cells.

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  • mRNA activity was further analyzed using sucrose density gradient centrifugation.

    • Main Results:

    • NDV stimulation induced both F and Le interferon production in fibroblasts, while poly(I)·poly(C) induced only F interferon.
    • mRNA from NDV-induced cells produced both F and Le interferons in oocytes, whereas mRNA from poly(I)·poly(C)-induced cells produced only F interferon.
    • The ratio of F and Le interferons produced in oocytes mirrored that in intact cells, suggesting mRNA-level regulation.
    • F and Le interferon mRNAs exhibited distinct sedimentation profiles, indicating differences in their molecular properties.

    Conclusions:

    • The differential synthesis of F and Le interferons in response to various stimuli is primarily regulated at the mRNA level.
    • The induction mechanisms for F and Le interferon mRNA synthesis are closely related but not identical.
    • These findings provide insights into the complex regulation of the interferon system and its implications for antiviral immunity.