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Interrelationship of reverse transcriptases of primate type D retroviruses
Archiv Fur Geschwulstforschung
|January 1, 1980
Summary
Antiserum to Mason-Pfizer monkey virus reverse transcriptase (RT) revealed commonalities among type D retroviruses. Relatedness of RTs does not correlate with structural protein relatedness across these retroviral isolates.
Area of Science:
- Virology
- Immunology
- Molecular Biology
Background:
- Type D retroviruses share common antigenic determinants on their reverse transcriptase (RT) enzymes.
- The RT of Mason-Pfizer monkey virus (MPMV) serves as a reference for comparative analysis.
Purpose of the Study:
- To investigate the antigenic relationships among reverse transcriptases of various type D retroviruses.
- To determine if antigenic relatedness of RTs correlates with the relatedness of major structural proteins.
Main Methods:
- Enzyme neutralization experiments using an antiserum specific to MPMV reverse transcriptase.
- Comparative analysis of antigenic determinants across different type D retroviral isolates.
Main Results:
- MPMV RT shares common antigenic determinants with RTs of all known type D retroviruses.
- MPMV RT is antigenically closely related to RTs from human-derived type D viruses, with detectable differences.
- MPMV RT shows a lesser antigenic relationship to squirrel monkey retrovirus (SMRV) and langur virus (PO-1-Lu) RTs.
- No correlation was observed between the antigenic relatedness of RTs and the relatedness of major structural proteins among type D viruses.
Conclusions:
- Reverse transcriptase enzymes of type D retroviruses exhibit conserved and distinguishing antigenic features.
- Antigenic relatedness of reverse transcriptase does not parallel the evolutionary relatedness of structural proteins in type D retroviruses.