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Age-related decrease in repair of oxidative damage to surface sulfhydryl groups on rat adipocytes.

B A Hughes, G S Roth, J Pitha

    Journal of Cellular Physiology
    |May 1, 1980
    PubMed
    Summary
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    Aging significantly reduces the ratio of surface sulfhydryl groups to total sulfhydryl and disulfide groups in rat adipocytes. This finding highlights age-related changes in cell surface chemistry.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Aging Research

    Background:

    • Adipocytes play crucial roles in energy metabolism and endocrine signaling.
    • Cell surface chemistry, particularly the state of sulfhydryl and disulfide groups, can influence cell function.
    • Age-related alterations in cellular components are a key area of aging research.

    Purpose of the Study:

    • To investigate the impact of aging on the surface sulfhydryl and disulfide groups of adipocytes.
    • To quantify age-dependent changes in the ratio of exofacial sulfhydryl to total exofacial sulfhydryl and disulfide groups in rat adipocytes.

    Main Methods:

    • Isolation of adipocytes from Wistar and Sprague-Dawley rats of varying ages.
    • Quantitation of surface sulfhydryl and disulfide groups using mercury-[3H] dextran, a cell-impermeable reagent.

    Related Experiment Videos

  • Pre-reduction of cells with 2-mercaptoethanol to measure total exofacial sulfhydryl and disulfide groups.

    • Main Results:

    • Aging significantly decreased the ratio of exofacial sulfhydryl groups to total exofacial sulfhydryl and disulfide groups in adipocytes.
    • This reduction was observed across different rat strains and ages studied.

    Conclusions:

    • Aging alters the redox state of the adipocyte cell surface.
    • The observed decrease in the sulfhydryl/disulfide ratio suggests a potential role in age-related metabolic dysfunction.