Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

alpha 1-Microglobulin from normal and pathological urines.

I Bernier, A Dautigny, B E Glatthaar

    Biochimica Et Biophysica Acta
    |November 20, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    School Achievement in Early Adolescence Is Associated With Students' Self-Perceived Executive Functions.

    Frontiers in psychology·2022
    Same author

    Sex differences in self-regulation in early, middle and late adolescence: A large-scale cross-sectional study.

    PloS one·2020
    Same author

    Modification of the stroop color word test improves differentiation between patients with mild head injury and matched controls.

    The Clinical neuropsychologist·2017
    Same author

    Event-related potentials and white matter lesions in bipolar disorder.

    Acta neuropsychiatrica·2016
    Same author

    Whiplash associated disorders: is there a place for psychiatrists and psychologists?

    Acta neuropsychiatrica·2016
    Same author

    Leeftijdsgeassocieerde cognitieve functiestoornissen: Cognitive aging in a longitudinal perspective: the Maastricht Aging Study (MAAS).

    Acta neuropsychiatrica·2016

    Alpha 1-microglobulin from normal and pathological urines exhibits distinct amino acid and charge heterogeneity. These differences in alpha 1-microglobulin composition and isoelectric points suggest unique structural variations between healthy and diseased states.

    Area of Science:

    • Biochemistry
    • Urine analysis

    Background:

    • Alpha 1-microglobulin (A1M) is a protein found in urine.
    • Variations in A1M may indicate physiological or pathological differences.

    Purpose of the Study:

    • To investigate the biochemical differences in alpha 1-microglobulin purified from normal and pathological urines.
    • To characterize the heterogeneity of A1M in different urine sources.

    Main Methods:

    • Purification of alpha 1-microglobulin from normal and pathological urine samples.
    • Analysis of amino acid composition.
    • Electrofocusing to determine isoelectric points and charge heterogeneity.
    • Determination of sugar composition and N-terminal sequences.

    Main Results:

    Related Experiment Videos

    • Significant differences in amino acid composition were observed between normal and pathological A1M.
    • Electrofocusing revealed distinct isoelectric points and intensity ratios for A1M from the two urine sources (normal: 3.8, 4.2; pathological: 4.2, 4.7).
    • Charge heterogeneity was noted in the second peaks from both sources, along with variations in sugar composition and N-terminal sequences.

    Conclusions:

    • Alpha 1-microglobulin isolated from pathological urine differs significantly from that of normal urine in terms of amino acid and sugar composition, charge heterogeneity, and isoelectric points.
    • These findings highlight structural variations in A1M associated with different urinary conditions.