Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

A procedure for detecting phosphonolipids on thin-layer chromatograms.

L W Stillway, S J Harmon

Journal of Lipid Research

|November 1, 1980

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Chemistry and antitumor evaluation of selected classical 2,4-diaminoquinazoline analogues of folic acid.

Journal of medicinal chemistry·1985

Same author

Improved synthesis and antitumor evaluation of 5,8-dideazaisofolic acid and closely related analogues.

Journal of medicinal chemistry·1984

Same author

Lipid composition of selected tissues of the hardshell clam, Mercenaria mercenaria.

Comparative biochemistry and physiology. B, Comparative biochemistry·1982

Same author

Leucine as an in vitro precursor to lipids in rat sciatic nerve.

Lipids·1979

Same author

A comparative circular dichroism study of relaxin and insulin.

Biochemical and biophysical research communications·1978

Same author

Leucine and isoleucine as in vitro precursors for lipid synthesis by rat aorta.

Lipids·1977

Same journal

The Metabolic-Immune Axis in MASH: Is the Macrophage Signature the Key to Liver Disease Staging?

Journal of lipid research·2026

Same journal

BK channel deficiency promotes lipolysis via an AKT-independent activation of the cAMP/PKA/HSL pathway.

Journal of lipid research·2026

Same journal

Interleukin-6 stimulates platelet 12-lipoxygenase to drive coagulation in inflammatory arthritis.

Journal of lipid research·2026

Same journal

LIPA regulates myeloid differentiation and is essential for intra-plaque macrophage accumulation during atherogenesis.

Journal of lipid research·2026

Same journal

Hydrolysis and depletion of phosphatidylglycerol at peak murine acute lung injury.

Journal of lipid research·2026

Same journal

Multispecies transcriptome analysis identifies networks protecting against MASLD in Göttingen minipigs.

Journal of lipid research·2026

See all related articles

A modified phospholipid spray enables specific detection of phosphonolipids and general lipid identification in thin-layer chromatography. This simple technique enhances lipid analysis for researchers.

Area of Science:

Biochemistry
Analytical Chemistry

Background:

Thin-layer chromatography (TLC) is a common technique for lipid separation.
Specific detection reagents are crucial for identifying different lipid classes.

Purpose of the Study:

To develop a modified spray reagent for enhanced lipid detection in TLC.
To enable specific identification of phosphonolipids alongside general lipid visualization.

Main Methods:

A phospholipid-specific spray reagent was modified.
The modified reagent was applied to thin-layer chromatograms.
Its efficacy for detecting phosphonolipids and general lipids was evaluated.

Main Results:

The modified spray served as a specific detection reagent for phosphonolipids.

Related Experiment Videos

The same reagent also functioned as a general lipid detection reagent.

The modification offers a dual-purpose application in lipid analysis.

Conclusions:

A simple modification to a phospholipid spray reagent significantly broadens its utility in TLC.
This enhanced reagent facilitates both specific phosphonolipid detection and general lipid visualization.
The findings offer a more efficient approach to lipid profiling using TLC.