[Passive hemagglutination reaction with adenovirus antigen]

In a passive hemagglutination reaction (micromethod for detecting IBP antibodies) were used 4 types of cellularly cultivated, group specific antigens from a cattle adenovirus, serotype 1. The most appropriate for the reaction proved to be the antigen obtained by a complete destruction of the infected cells and deduction of the soluble viral hexen components. The production of th antibodies does not require a special equipment. A high-titre antigen is obtained, which can be well loaded passively on tanninized sheep erythrocytes. A much higher sensitivity of passive hemagglutination reaction was obtained in comparison with virus neutralizing reaction and immunodiffusion reaction. Therefore, for mass serological investigations if serums from calves suffering from bronchial pneumonia, a much larger quantity of seroreagents are detected, which remain undetected if immunodiffusion reaction is applied. Prospects are discussed for the implementation of this micromethod for mass serological investigations in detecting the dissemination of adenoviral infections with cattle.