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Erythro-immunoassay using phytohaemagglutinin "phaseolosaxin"
Annales D'Immunologie
|January 1, 1981
Summary
Phytohaemagglutinin (PHA) can be used in lectin immunoassays to quantify antigens and antibodies. This method offers greater sensitivity compared to ConA, expanding options for erythro-adsorption immunoassays.
Area of Science:
- Biochemistry
- Immunology
- Biotechnology
Background:
- Lectin-based immunoassays offer versatile tools for molecular detection.
- Glutaraldehyde is a common cross-linking agent for conjugating biomolecules.
Purpose of the Study:
- To investigate the utility of phytohaemagglutinin (PHA) in lectin immunoassays.
- To evaluate PHA's effectiveness in quantifying specific antigens and antibodies.
Main Methods:
- Coupling of antigens and antibodies to lectins using glutaraldehyde.
- Quantification of human alpha-foetoprotein and anti-DNA/anti-actin antibodies in patient serum.
- Comparison of PHA with other lectins, such as ConA, for assay sensitivity.
Main Results:
- Two PHA preparations were found suitable for lectin immunoassays.
- PHA demonstrated higher sensitivity than ConA in the tested immunoassays.
- Successful quantification of target analytes was achieved using PHA conjugates.
Conclusions:
- Phytohaemagglutinin (PHA) is a viable lectin for use in immunoassays.
- PHA enhances sensitivity in erythro-adsorption based quantification of antigens and antibodies.
- PHA expands the repertoire of lectins available for immunoassay development.