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Related Experiment Videos

Collodion membrane secures cells sorted by flow cytofluorometry onto microscope slides.

R A Meck, N A Benson, A G Ng

    Cytometry
    |July 1, 1980
    PubMed
    Summary

    A collodion membrane preserves nearly all cells sorted for quantitative microscopic cytology. This technique overcomes cell loss during staining, improving slide preparation for flow cytofluorometry analysis.

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    Area of Science:

    • Cytopathology
    • Cell Biology
    • Biotechnology

    Background:

    • Quantitative microscopic cytology relies on accurate cell counts.
    • Cell loss during slide preparation, particularly staining, is a significant challenge.
    • Flow cytofluorometry sorting requires high cell retention for reliable analysis.

    Purpose of the Study:

    • To develop a method to prevent cell loss during staining for quantitative microscopic cytology.
    • To improve cell retention on microscope slides after flow cytofluorometry sorting.
    • To enhance the reliability of quantitative microscopic cytology and flow cytofluorometry.

    Main Methods:

    • Cells were sorted directly onto microscope slides.
    • A semi-permeable collodion membrane was applied over fixed or unfixed cells.

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  • Cells were stained using Papanicolaou's stain and routine clinical cervical cytologic preparations.
  • Comparison of cell retention on collodion-coated versus uncoated/albumin-coated slides.

    • Main Results:

    • Application of a collodion membrane secured virtually 100% of sorted cells on the slide.
    • Slides coated with collodion showed significantly higher cell retention compared to uncoated or albumin-coated slides.
    • Fewer than half of the cells were retained on slides without the collodion membrane after staining.

    Conclusions:

    • A simple collodion membrane application effectively prevents cell loss during staining procedures.
    • This method significantly improves cell retention for quantitative microscopic cytology.
    • The technique enhances the accuracy and reliability of analyses involving sorted cells, including flow cytofluorometry.