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Factor XII assay with the chromogenic substrate chromozym PK
Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
|June 1, 1981
Summary
A new chromogenic assay accurately measures factor XII (coagulation factor) levels. This method identified reduced factor XII activity in deep vein thrombosis patients.
Area of Science:
- Clinical Chemistry
- Hematology
- Coagulation Science
Background:
- Factor XII (FXII) plays a role in the intrinsic pathway of blood coagulation.
- Accurate measurement of FXII activity is crucial for diagnosing hemostatic disorders.
Purpose of the Study:
- To evaluate a novel chromogenic assay for quantifying factor XII activity.
- To establish normal FXII ranges and assess its levels in deep vein thrombosis (DVT) patients.
Main Methods:
- A chromogenic assay utilizing Chromozym PK substrate was developed and validated.
- Assay linearity, normal ranges (n=50), and correlation with a clotting assay were determined.
- FXII levels were assessed in patients diagnosed with deep vein thrombosis.
Main Results:
- The chromogenic assay demonstrated linearity from 10 U/l to over 200 U/l.
- Normal factor XII levels were established at 136 U/l ± 27 U/l.
- A strong correlation (r=0.831) was observed between the chromogenic and clotting assays.
- Patients with deep vein thrombosis exhibited approximately 60% of normal factor XII activity.
Conclusions:
- The evaluated chromogenic assay is a reliable method for determining factor XII activity.
- Reduced factor XII levels may be associated with deep vein thrombosis.
- This assay provides a valuable tool for hemostasis research and clinical diagnostics.