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Related Experiment Videos

Cell cultures producing human interferon.

P C Familletti, S Pestka

    Antimicrobial Agents and Chemotherapy
    |July 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Most human leukocyte cell lines produce interferon, a key antiviral protein. Some cell lines, particularly B-lymphoblasts, showed high interferon production after Newcastle disease virus induction.

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    Area of Science:

    • Immunology
    • Virology
    • Cell Biology

    Background:

    • Interferons (IFNs) are crucial proteins in the innate immune response against viral infections.
    • Leukocyte-derived cell lines are valuable tools for studying cellular responses and producing therapeutic proteins.
    • Understanding the interferon-producing capacity of different cell types is important for antiviral research.

    Purpose of the Study:

    • To investigate the potential of human leukocyte-derived cell lines, specifically B-lymphoblasts, to produce human interferon.
    • To determine if interferon production is constitutive or inducible in these cell lines.
    • To identify cell lines capable of producing high levels of interferon.

    Main Methods:

    • Culturing of 27 B-lymphoblast cell lines and 5 other human leukocyte-derived cell lines.

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  • Induction of interferon production using Newcastle disease virus (NDV).
  • Quantification of human interferon production in cell culture supernatants.

    • Main Results:

    • The majority of tested B-lymphoblast and leukocyte cell lines exhibited interferon production.
    • Interferon production was observed both constitutively (without induction) and after induction with NDV.
    • Several cell lines demonstrated relatively high levels of interferon production following NDV induction.

    Conclusions:

    • Human leukocyte-derived cell lines, especially B-lymphoblasts, are a viable source for producing human interferon.
    • Induction with Newcastle disease virus can significantly enhance interferon yields in susceptible cell lines.
    • These findings support the use of specific leukocyte cell lines for research and potential therapeutic applications of interferons.