[Relative hydrophobicity of mononucleotides and nucleosides determined by partition in aqueous two-phase polymeric...

The effect of ionic strength on the partition of several mononucleotides, deoxyribonucleosides and the corresponding bases in aqueous buffered (pH 7.4) ficoll--dextran biphasic system was examined. The relative hydrophobicity of the compounds at zero ionic strength was estimated in terms of an equivalent number of CH2 groups. It is found that the effect of the ionic strength on the relative hydrophobicity of the phosphate group is similar for all the mononucleotides examined but for the adenosine derivative.