Related Experiment Video

Updated: Aug 19, 2026

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening
08:34

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

Published on: October 16, 2015

A rapid and sensitive method for determination of covalent binding of benzo[a]pyrene to proteins

H Wallin, C Schelin, A Tunek

    Chemico-Biological Interactions
    |December 1, 1981
    PubMed

    More Related Videos

    Characterization, Quantification and Compound-specific Isotopic Analysis of Pyrogenic Carbon Using Benzene Polycarboxylic Acids (BPCA)08:12

    Characterization, Quantification and Compound-specific Isotopic Analysis of Pyrogenic Carbon Using Benzene Polycarboxylic Acids (BPCA)

    Published on: May 16, 2016

    Detection of CD40 Protein-Umbelliferone Interaction via Differential Scanning Fluorescence05:30

    Detection of CD40 Protein-Umbelliferone Interaction via Differential Scanning Fluorescence

    Published on: March 1, 2024

    Related Concept Videos

    NMR Spectroscopy of Benzene Derivatives01:37

    NMR Spectroscopy of Benzene Derivatives

    Simple unsubstituted benzene has six aromatic protons, all chemically equivalent. Therefore, benzene exhibits only a singlet peak at δ 7.3 ppm in the 1H NMR spectrum. The observed shift is far downfield because the aromatic ring current strongly deshields the protons. Any substitution on the benzene ring makes the aromatic protons nonequivalent, and the protons split each other. The peak is, therefore, no longer a singlet and the splitting pattern and their associated coupling constants depend...
    Electrophilic Aromatic Substitution: Fluorination and Iodination of Benzene01:13

    Electrophilic Aromatic Substitution: Fluorination and Iodination of Benzene

    Bromination and chlorination of aromatic rings by electrophilic aromatic substitution reactions are easily achieved, but fluorination and iodination are difficult to achieve. Fluorine is so reactive that its reaction with benzene is difficult to control, resulting in poor yields of monofluoroaromatic products. To address this, Selectfluor reagent is used as a fluorine source in which a fluorine atom is bonded to a positively charged nitrogen.
    Mass Spectrometry: Aromatic Compound Fragmentation01:23

    Mass Spectrometry: Aromatic Compound Fragmentation

    Upon ionization, aromatic compounds generate a molecular ion that is observed as a prominent peak in their mass spectra. For example, the molecular ion peak for benzene appears at a mass-to-charge ratio of 78, while toluene is observed at a mass-to-charge ratio of 92. The molecular ion benzene is highly stable and does not readily undergo further fragmentation due to the significant amount of energy required to disrupt the aromatic stability of the benzene ring. In contrast, the molecular ion...
    Protein-Drug Binding: Determination Methods01:22

    Protein-Drug Binding: Determination Methods

    Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
    Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...